Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration.
Identifieur interne : 000000 ( Main/Exploration ); suivant : 000001Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration.
Auteurs : César Bernat-Silvestre [Espagne] ; Vanessa De Sousa Vieira [Royaume-Uni] ; Judit Sánchez-Simarro [Espagne] ; Fernando Aniento [Espagne] ; María Jesús Marcote [Espagne]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2021.
Abstract
Transient expression in Arabidopsis thaliana seedlings allows fast expression of fluorescent markers for different subcellular compartments. This protocol describes a transient transformation assay with five-day-old seedlings using Agrobacterium tumefaciens-mediated vacuum infiltration. Three days after infiltration of the Agrobacterium containing an expression vector for a fluorescent marker of interest, cotyledon cells expressing the fluorescent protein can be imaged in a confocal microscope. This assay allows high-throughput screening of new constructs and the study of the localization of a large number of subcellular markers in Arabidopsis seedlings including wild-type, stable over-expressing and mutant lines.
DOI: 10.1007/978-1-0716-0880-7_6
PubMed: 33175376
Affiliations:
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This protocol describes a transient transformation assay with five-day-old seedlings using Agrobacterium tumefaciens-mediated vacuum infiltration. Three days after infiltration of the Agrobacterium containing an expression vector for a fluorescent marker of interest, cotyledon cells expressing the fluorescent protein can be imaged in a confocal microscope. This assay allows high-throughput screening of new constructs and the study of the localization of a large number of subcellular markers in Arabidopsis seedlings including wild-type, stable over-expressing and mutant lines.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33175376</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>2200</Volume><PubDate><Year>2021</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration.</ArticleTitle><Pagination><MedlinePgn>147-155</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-0716-0880-7_6</ELocationID><Abstract><AbstractText>Transient expression in Arabidopsis thaliana seedlings allows fast expression of fluorescent markers for different subcellular compartments. This protocol describes a transient transformation assay with five-day-old seedlings using Agrobacterium tumefaciens-mediated vacuum infiltration. Three days after infiltration of the Agrobacterium containing an expression vector for a fluorescent marker of interest, cotyledon cells expressing the fluorescent protein can be imaged in a confocal microscope. This assay allows high-throughput screening of new constructs and the study of the localization of a large number of subcellular markers in Arabidopsis seedlings including wild-type, stable over-expressing and mutant lines.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bernat-Silvestre</LastName><ForeName>César</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina (ERI BIOTECMED), Universitat de València, Burjassot (Valencia), Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De Sousa Vieira</LastName><ForeName>Vanessa</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Faculty of Health and Life Sciences, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sánchez-Simarro</LastName><ForeName>Judit</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina (ERI BIOTECMED), Universitat de València, Burjassot (Valencia), Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aniento</LastName><ForeName>Fernando</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina (ERI BIOTECMED), Universitat de València, Burjassot (Valencia), Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marcote</LastName><ForeName>María Jesús</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular, Estructura de Recerca Interdisciplinar en Biotecnología i Biomedicina (ERI BIOTECMED), Universitat de València, Burjassot (Valencia), Spain. mariajesus.marcote@uv.es.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Agrobacterium tumefaciens</Keyword><Keyword MajorTopicYN="Y">Arabidopsis thaliana</Keyword><Keyword MajorTopicYN="Y">Fluorescent markers</Keyword><Keyword MajorTopicYN="Y">Seedlings transformation</Keyword><Keyword MajorTopicYN="Y">Vacuum infiltration</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>12</Hour><Minute>15</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33175376</ArticleId><ArticleId IdType="doi">10.1007/978-1-0716-0880-7_6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Bernat Silvestre, Cesar" sort="Bernat Silvestre, Cesar" uniqKey="Bernat Silvestre C" first="César" last="Bernat-Silvestre">César Bernat-Silvestre</name></noRegion><name sortKey="Aniento, Fernando" sort="Aniento, Fernando" uniqKey="Aniento F" first="Fernando" last="Aniento">Fernando Aniento</name><name sortKey="Marcote, Maria Jesus" sort="Marcote, Maria Jesus" uniqKey="Marcote M" first="María Jesús" last="Marcote">María Jesús Marcote</name><name sortKey="Sanchez Simarro, Judit" sort="Sanchez Simarro, Judit" uniqKey="Sanchez Simarro J" first="Judit" last="Sánchez-Simarro">Judit Sánchez-Simarro</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="De Sousa Vieira, Vanessa" sort="De Sousa Vieira, Vanessa" uniqKey="De Sousa Vieira V" first="Vanessa" last="De Sousa Vieira">Vanessa De Sousa Vieira</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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